The Kuban State Medical University
Krasnodar, Krasnodar, Russian Federation
The Kuban State Medical University
Krasnodar, Krasnodar, Russian Federation
The Kuban State Medical University
Krasnodar, Krasnodar, Russian Federation
The paper presents a comparative analysis of the degree of oxidative DNA damage in epidermolysis bullosa (EB) and bronchial asthma (BA). The degree of oxidative damage to DNA was assessed by the level of 8-oxoguanine (8-oxoG) concentration in blood serum, determined by enzyme immunoassay with monoclonal antibodies. It was found that the concentration of the modified base 8-oxoG in patients with BE is 2.1 times higher than in the control group. In BA, this indicator changes insignificantly compared to the control. Different concentrations of 8-oxoG in BE and BE indicate the severity of structural DNA damage in BE and the almost absence of oxidative DNA modification in AD, which may indicate different mechanisms of pathophysiological disorders in these nosologies at the cellular level. The content of 8-oxoG in the blood DNA of healthy donors and patients with BE and AD was determined after exposure to an alternating magnetic field (MF) of (550 ± 30) A/m in the frequency range from 3 to 60 Hz in vitro. It was shown that, after MP treatment, there was a significant increase in the levels of 8-oxoG in DNA for both groups, which depended in a complex way on frequency. The effect obtained is explained by the generation of ROS under the influence of magnetic fields and the disruption of DNA repair processes. An analysis of the association of polymorphic variants of the rs652438 locus of the mmp12 gene in AD was carried out. The presence of significant differences in the frequency of heterozygotes was shown. In the control group, this figure is 2.3 more than in BA. The G allele frequency in the group of healthy donors was 0.15, in patients with AD - 0.06. The value of the odds ratio indicates that the influence of the minor allele G is protective in nature, reducing the risk of developing AD for its owners.
epidermolysis bullosa, bronchial asthma, oxidative DNA damage, 8-oxoguanine, MMP-12 gene polymorphism
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