Nizhny Novgorod, Nizhny Novgorod, Russian Federation
Combined thermal trauma (CTT), including thermal inhalation exposure and burns of the skin, is often found in victims entering burn centers. The aim of the study was to study the peculiarities of regulation of antioxidant protection enzymes in the blood during experimental CTT under the influence of NO. The experiment was carried out on 30 white male rats of the Wistar line. The animals were divided into 3 groups of equal numbers: 1 – intact rats; 2 – control – animals with CTT; 3 – experimental – animals with CTT who received inhalation NO. Rats under anesthesia were subjected to contact burn (20% of the body surface) and thermal inhalation exposure. The animals were removed from the experiment after CTT for 10 days. The activity of superoxide dimsutase (SOD), catalase, glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (Gl-6-fDh) was determined in red blood cells. Inhalation-external exposure to NO during CTT was carried out daily for 10 days for 10 minutes. Kinetic parameters of the enzymatic reaction were calculated. Statistical processing of the results was carried out using Statistica 6.0 (Statsoft Inc., USA). It was shown that against the background of a decrease in the activity of antioxidant enzymes in the blood during CTT, the greatest inhibition was noted for catalase. The kinetic method of analysis for the first time established the nature of enzyme inhibition in CTT: for SOD and catalase in erythrocytes – two-parametrically consistent, for GR and Gl-6–fDh - catalytic. Inhalations of NO during CTT activated SOD, catalase, GR and Gl-6-fDh.
combined thermal injury, nitric oxide, antioxidant protection enzymes
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