WAYS OF REALIZATION OF APOPTOSIS OF HUMAN LYMPHOCYTES INDUCED BY HYDROGEN PEROXIDE AND THEIR REGULATION

M. Nakvasina; V. Artyukhov; T. Starikova; N. Saradzhi

doi:10.29039/rusjbpc.2022.0541

UDC

577

WAYS OF REALIZATION OF APOPTOSIS OF HUMAN LYMPHOCYTES INDUCED BY HYDROGEN PEROXIDE AND THEIR REGULATION

Published in Russian Journal of Biological Physics and Chemisrty · Pages 440–445 · Rubric: MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY

DOI: https://doi.org/10.29039/rusjbpc.2022.0541

Received: 20.09.2022 Accepted: 20.09.2022 Published: 28.09.2022

Authors

M. Nakvasina

V. Artyukhov

T. Starikova

N. Saradzhi

M. Nakvasina

Voronezh State University Russian Federation · Voronezh

V. Artyukhov

Voronezh State University Russian Federation · Voronezh

T. Starikova

Voronezh State University Russian Federation · Voronezh

N. Saradzhi

Voronezh State University Russian Federation · Voronezh

ABSTRACT

The changes in marker indicators of apoptotic cell death were studied: the level of functional activity of initiating caspases-8, -9, -12, membrane death receptors Fas, intracellular reactive oxygen species, cytosolic calcium, mitochondrial potential of peripheral blood lymphocytes of donors induced by exposure to hydrogen peroxide at final concentrations of 10-6 and 10-5 mol/l. Activation of initiating caspase-8 associated with membrane death receptors Fas was detected after exposure to hydrogen peroxide lymphocytes at a concentration of 10-6 mol/l. There was a significant increase in the functional activity of initiating caspase-9, the level of Fas receptors and intracellular reactive oxygen species, as well as a decrease in the concentration of cytosolic calcium and the value of the mitochondrial membrane potential 1 and 2 hours after modification of cells with hydrogen peroxide at a concentration of 10-5 mol/l compared with those for intact immunocytes. It was found that apoptosis of lymphocytes under the influence of exogenous hydrogen peroxide is realized with the participation of receptor-mediated caspase, mitochondrial (with activation of caspase-9) and p53-dependent mechanisms. The protective effect of resveratrol (10-6 mol/l) on lymphocytes after exposure to hydrogen peroxide (10-5 mol / l), which is due to its ability to reduce the level of reactive oxygen species in intact and modified hydrogen peroxide immunocytes, as well as deactivate hydrogen peroxide.

KEYWORDS

apoptosis, lymphocytes, hydrogen peroxide, caspases

Funding

Rabota vypolnena pri podderzhke Ministerstva nauki i vysshego obrazovaniya RF v ramkah gosudarstvennogo zadaniya VUZam v sfere nauchnoy deyatel'nosti na 2020—2022 gody, proekt № FZGU-2020-0044.

1. Nagata S., Masato Tanaka. Programmed cell death and the immune system. Nature Reviews Immunology, 2017, vol. 17, no. 5, pp. 333-340, doi:https://doi.org/10.1038/nri.2016.153.

2. Savitskaya M.A., Onishchenko G.E. Mechanisms of Apoptosis. Biochemistry (Mosc.), 2015, vol. 80, no. 11, pp. 1393-1405, doi:https://doi.org/10.1134/S0006297915110012. EDN: https://elibrary.ru/VALWUF

3. Nakvasina M.A., Artyukhov V.G. Mechanisms of cell death: apoptosis, autophagy, necrosis. Textbook. Voronezh: VSU Publishing House, 2019, 164 p. (In Russ.) EDN: https://elibrary.ru/OXYEUA

4. Mates J.M., Sanchez-Jimenez F.M. Role of reactive oxygen species in apoptosis: implications for cancer therapy. The International Jornal of Biochemistry & Cell Biology, 2000, vol. 32, pp. 157-170, doi:https://doi.org/10.1016/s1357-2725(99)00088-6.

5. Riazantseva N.V., Novitskii V.V., Chasovskikh N.Iu., Kaigorodova E.V., Starikova E.G., Starikov Iu.V., Radzivil T.T., Krat N.V. The role of redox-dependent signal systems in the regulation of apoptosis under oxidative stress condition. Tsitologiia, 2009, vol. 51, no. 4, pp. 329-334. (In Russ.) EDN: https://elibrary.ru/OJLAUZ

6. Cherenkevich S.N., Martinovich G.G., Martinovich I.V., Gorudko I.V., Shamova E.V. Redox regulation of cellular activity: concepts and mechanisms. Vescі NAN Belarusі. Ser. biyal. Navuk, 2013, vol. 1, pp. 92-108. (In Russ.)

7. Nadeev A.D., Zinchenko V.P., Kudryavtsev I.V., Serebriakova M.K., Avdonin P.V., Goncharov N.V. Dual proapoptptic and pronecrotic effect of hydrogen peroxide on human umbilical vein endothelial cells. Cell and Tissue Biology, 2015, vol. 57, no. 12, pp. 909-916, doi:https://doi.org/10.1134/S1990519X16020097. EDN: https://elibrary.ru/VCOJET

8. Artyukhov V.G., Trubitsyna M.S., Nakvasina M.A., Solov′eva E.V., Lidokhova O.V. Ways of realizing apoptosis of human lymphocytes induced by UV-light and reactive oxygen species. Radiats Biol Radioecol, 2011, vol. 51, no. 4, pp. 425-443. (In Russ.) EDN: https://elibrary.ru/OCXOJR

9. Artyukhov V.G., Trubitsyna M.S., Nakvasina M.A., Solov′eva E.V. DNA Fragmentation of Human Lymphocytes in Dynamics of Development of Apoptosis Induced by Action of UV Radiation and Reactive Oxygen Species. Cell and Tissue Biology, 2011, vol. 5, no. 2, pp. 127-135, doi:https://doi.org/10.1134/S1990519X11020039. EDN: https://elibrary.ru/OIBFWT

10. Artyukhov V.G., Trubitsyna M.S., Nakvasina M.A., Lidokhova O.V., Ryazantsev S.V. The ways for realization of human lymphocyte apoptosis, induced by hydrogen peroxide. Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii, 2013, no. 7, pp. 7-16. (In Russ.) EDN: https://elibrary.ru/PHCOJN

11. Nakvasina M.A., Popova L.I., Lidokhova O.V., Artyukhov V.G. Modulation of Structural and Functional Properties of Human Lymphocytes by Reactive Oxygen Species. Bulletin of Experimental Biology and Medicine, 2019. vol. 166, no. 4, pp. 481-486, doi:https://doi.org/10.1007/S10517-019-04377-4. EDN: https://elibrary.ru/WWFEOM

12. Klaus J. Lymphocytes. Methods. Moscow, Mir, 1990, 395 p. (In Russ.)

13. Kaufmann S., Lee Sun-Hee, Meng X., Loegering D., Kottke T., Heinzing A.J., Ruchaud S., Samejima K., Earnshaw W. Apoptosis-associated caspase activation assays. Methods, 2008, vol. 44, pp. 262-272, doi:https://doi.org/10.1016/j.ymeth.2007.11.005.

14. Hirst R.A., Harrison C., Hirota K., Lambert D.G. Measurement of [Ca²+]i in whole cell suspensions using fura-2. Methods Mol. Biol., 2005, vol. 312, pp. 37-45, doi:https://doi.org/10.1385/1-59259-949-4:037.

15. Rastogi R.P., Singh S.P., Hader D.P., Sinha R.P. Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2',7'-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937. Biochem. Biophys. Res. Commun., 2010, vol. 397, no. 3, pp. 603-607, doi:https://doi.org/10.1016/j.bbrc.2010.06.006. EDN: https://elibrary.ru/NWKXFV

16. Kudriavtsev I.V., Golovkin A.S., Zurochka A.V., Khaidukov S.V. Modern technologies and approaches to apoptosis studies in experimental biology. Medicinskaya immunologiya, 2012, vol. 14, no. 6, pp. 461-482. (In Russ.) EDN: https://elibrary.ru/PESFXJ

17. Julien O., Wells I.A. Caspases and their substrates. Cell Death and Differentiation. Advance online publication. 2017, doi: 10/1038/cdd.2017.44.

18. Green D.R., Llambi F. Cell death signaling. Cold Spring Harb Perspect Biol., 2015, doi:https://doi.org/10.1101/cshperspect.a006080.

19. Szegezdi E., Fitzgerald U., Samali A. Caspase-12 and ER-stress-mediated apoptosis: The story so far. Annals of the New York Academy of Sciences, 2003, dec., vol. 1010, pp. 186-194, doi:https://doi.org/10.1196/annals.1299.032.

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Dmitriy Voronin

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Chief Editor

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Science Editor

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This work is licensed under Creative Commons Attribution 4.0 International Creative Commons

GOST

Nakvasina M. A., Artyukhov V. G., Starikova T. I., Saradzhi N. G. WAYS OF REALIZATION OF APOPTOSIS OF HUMAN LYMPHOCYTES INDUCED BY HYDROGEN PEROXIDE AND THEIR REGULATION // . 2022. pp. 440-445. DOI: https://doi.org/10.29039/rusjbpc.2022.0541. (Date of access 20.08.2026).

MLA

M. Nakvasina, Artyukhov V. G., Starikova T. I., Saradzhi N. G.. "WAYS OF REALIZATION OF APOPTOSIS OF HUMAN LYMPHOCYTES INDUCED BY HYDROGEN PEROXIDE AND THEIR REGULATION." (2022): 440-445. DOI: https://doi.org/10.29039/rusjbpc.2022.0541.

APA

Nakvasina M. A., Artyukhov V. G., Starikova T. I., Saradzhi N. G. (2022). WAYS OF REALIZATION OF APOPTOSIS OF HUMAN LYMPHOCYTES INDUCED BY HYDROGEN PEROXIDE AND THEIR REGULATION. , 440-445. https://doi.org/10.29039/rusjbpc.2022.0541

Chicago

M. Nakvasina, Artyukhov V. G., Starikova T. I., Saradzhi N. G.. "WAYS OF REALIZATION OF APOPTOSIS OF HUMAN LYMPHOCYTES INDUCED BY HYDROGEN PEROXIDE AND THEIR REGULATION." , no. (2022): 440-445. https://doi.org/10.29039/rusjbpc.2022.0541.

WIKI

{{cite journal | author = Nakvasina M. A., Artyukhov V. G., Starikova T. I., Saradzhi N. G. | title = WAYS OF REALIZATION OF APOPTOSIS OF HUMAN LYMPHOCYTES INDUCED BY HYDROGEN PEROXIDE AND THEIR REGULATION | journal = | year = 2022 | pages = 440-445 | doi = 10.29039/rusjbpc.2022.0541 }}

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