С использованием метода фиксации потенциала исследовали участие сигма-1 рецепторов в регуляции глутоксимом транспорта Na+ в коже лягушки. Показано, что обработка кожи лягушки антагонистом рецепторов сигма-1 аминазином подавляет стимулирующее влияние глутоксима на транспорт Na+. Полученные результаты свидетельствуют о возможном участии рецепторов сигма-1 в регуляции глутоксимом транспорта Na+ в коже лягушки.
кожа лягушки, трансэпителиальный транспорт Na+, глутоксим, аминазин
1. Наточин Ю.В. Основы физиологии почки. Л.: Наука, 1982, 184 с. [Natochin, Yu.V. Fundamentals of kidney physiology. Leningrad: Nauka, 1982. 184 p.]
2. Boldyrev A.A., Bulygina E.R. Na/K-ATPase and oxidative stress. Ann. N.Y. Acad. Sci., 1997, vol. 834, pp. 666-668.
3. Firsov D., Robert-Nicoud M., Gruender S., Schild L., Rossier B.C. Mutational analysis of cysteine-rich domain of the epithelium sodium channel (ENaC): Identification of cysteines essential for channel expression at the cell surface. J. Biol. Chem., 1999, vol. 274, рр. 2743-2749.
4. Krutetskaya Z.I., Lebedev O.E., Melnitskaya A.V., Antonov V.G., Nozdrachev A.D. Effect of disulfide containing compounds on Na+ transport in frog skin. Dokl. Akad. Nauk, 2008, vol. 421, no. 5, pp. 709-712.
5. Melnitskaya A.V., Krutetskaya Z.I., Lebedev O.E., Antonov V.G., Butov S.N. Involvement of tyrosine and phosphatidylinositol kinases in oxidized glutathione and glutoxim regulation of Na+ transport in frog skin. Cell and Tissue Biology, 2010, vol. 4, no. 3, pp. 273-279.
6. Melnitskaya A.V., Krutetskaya Z.I., Lebedev O.E., Butov S.N., Krutetskaya N.I., Antonov V.G. The effect of glutoxim on Na+ transport in frog skin: the role of cytoskeleton. Cell and Tissue Biology, 2012, vol. 6, no. 3, рр. 248-253.
7. Krutetskaya Z.I., Melnitskaya A.V., Antonov V.G., Nozdrachev A.D. Inhibitors of the cyclooxygenase oxidation pathway of arachidonic acid suppress the stimulating effect of glutoxim on Na+ transport in frog skin. Dokl. Biol. Sci., 2013, vol. 451, no. 1, pp. 193-195.
8. Krutetskaya Z.I., Melnitskaya A.V., Antonov V.G., Nozdrachev A.D. The inhibitors of Arp2/3 complex and WASP proteins modulate the effect of glutoxim on Na+ transport in frog skin. Dokl. Biochem. Biophys., 2016, vol. 467, pp. 102-104.
9. Krutetskaya Z.I., Melnitskaya A.V., Antonov V.G., Nozdrachev A.D. Lipoxygenases modulate the effect of glutoxim on Na+ transport in the frog skin epithelium. Dokl. Biochem. Biophys., 2017, vol. 474, pp. 193-195.
10. Krutetskaya Z.I., Melnitskaya A.V., Antonov V.G., Nozdrachev A.D. Epoxygenase inhibitors attenuate the stimulatory effect of glutoxim on Na+ transport in frog skin. Dokl. Biochem. Biophys., 2018, vol. 480, pp. 1-3.
11. Benos D.J., Stanton B.A. Functional domains within the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. J. Physiol., 1999, vol. 520, pp. 631-644.
12. Kellenberger S., Schild L. Epithelial sodium channel/Degenerin family of ion channels: a variety of function for a shared structure. Physiol. Rev., 2002, vol. 82, pp. 735-767.
13. Rousseaux C.G., Greene S.F. Sigma receptors [σRs]: biology in normal and diseased states. J. Recept. Signal. Trans., 2016, vol. 36, pp. 327-388.
14. Hellewell S.B., Bruce A., Feinstein G., Orringer J., Williams W., Bowen W.D. Rat liver and kidney contain high densities of sigma 1 and sigma 2 receptors: characterization by ligand binding and photoaffinity labeling. Eur. J. Pharmacol., 1994, vol. 268, pp. 9-18.
15. Su T.-P., Hayashi T., Maurice T., Buch S., Ruoho A.E. The sigma-1 receptor chaperone as an inter-organelle signaling modulator. Trends Pharmacol. Sci., 2010, vol. 31, pp. 557566.
16. Cobos E.J., Entrena J.M., Nieto F.R., Cendán C.M, Del Pozo E. Pharmacology and therapeutic potential of sigma(1) receptor ligands. Curr. Neuropharmacol., 2008, vol. 6, pp. 344-366.
17. Мельницкая А.В., Крутецкая З.И., Бутов С.Н., Крутецкая Н.И., Антонов В.Г. Влияние нейролептиков на транспорт Na+ в коже лягушки. Актуальные вопросы биологической физики и химии. БФФХ-2017, Севастополь, 2017, т.1, с. 272-275. [Melnitskaya A.V., Krutetskaya Z.I., Butov S.N., Krutetskaya N.I., Antonov V.G. The effect of neuroleptics on Na+ transport in frog skin. Modern trends in biological physics and chemistry. BPPC-2017, Sevastopol, 2017, vol. 1, pp. 272-275 (In Russ.])
18. Itzhak Y., Ruhland M., Krahling H. Binding of umespirone to the sigma receptor: evidence for multiple affinity states. Neuropharmacol., 1990, vol. 29, pp. 181-184.
19. Carnally S.M., Johannessen M., Henderson R.M., Jackson M.B., Edwardson J.M. Demonstration of a direct interaction between σ-1 receptors and acid-sensing ion channels. Biophys. J., 2010, vol.98, pp. 1182-1191.
20. Herrera Y., Katnik C., Rodriguez J.D., Hall A.A., Willing A., Pennypacker K.R., Cuevas J. Sigma-1 receptor modulation of acid-sensing ion channel a (ASIC1a) and ASIC1a-induced Ca2+ influx in rat cortical neurons. J. Pharmacol. Exp. Ther., 2008, vol. 327, pp. 491-502.
