Красноярск, Красноярский край, Россия
С помощью адсорбции экстраклеточных оксидаз (глюкозооксидаза и пероксидазы) светящегося гриба Neonothopanus nambi на модифицированные наноалмазы (МНА) детонационного синтеза сконструирована бифункциональная индикаторная система для обнаружения глюкозы и фенола в водной среде. Установлено, что изучаемые ферменты прочно связываются частицами МНА и проявляют после этого каталитическую активность. В модельных экспериментах in vitro показано, что полученная индикаторная система (комплекс МНА-ферменты) может многократно использоваться для тестирования глюкозы и фенола и проявляет функциональную активность в течение длительного (до 1 месяца) хранения при 4 °С. Полученные данные открывают перспективы конструирования на основе частиц МНА и ферментов высших грибов нового класса многоразовых индикаторных тест-систем для медицинской и экологической аналитики.
наноалмазы, светящийся гриб, мицелий, β-глюкозидаза, глюкозооксидаза, пероксидаза, индикаторная система
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