Krasnoyarsk, Krasnoyarsk, Russian Federation
A bifunctional indicator system for glucose and phenol detection has been constructed by adsorption of extracellular oxidases (glucose oxidase and peroxidases) of luminous fungus Neonothopanus nambi onto modified nanodiamonds (MND) synthesized by detonation. It has been found that the studied enzymes are strongly bound by MND particles and exhibit catalytic activity. Model experiments in vitro have shown that created indicator system (MND-enzymes complex) can be repeatedly used for glucose and phenol testing and exhibit their activity after storage at 4 °C for a long time (up to 1 month). The data obtained open the prospects for developing a new class of reusable multifunctional indicating and diagnostic test-systems on the basis of MND particles and higher fungi enzymes for medical and ecological analytics.
nanodiamonds, luminous fungus, mycelium, β-glucosidase, glucose oxidase, peroxidase, indicator system
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