Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
Voronezh, Voronezh, Russian Federation
The changes in marker indicators of apoptotic cell death were studied: the level of functional activity of initiating caspases-8, -9, -12, membrane death receptors Fas, intracellular reactive oxygen species, cytosolic calcium, mitochondrial potential of peripheral blood lymphocytes of donors induced by exposure to hydrogen peroxide at final concentrations of 10-6 and 10-5 mol/l. Activation of initiating caspase-8 associated with membrane death receptors Fas was detected after exposure to hydrogen peroxide lymphocytes at a concentration of 10-6 mol/l. There was a significant increase in the functional activity of initiating caspase-9, the level of Fas receptors and intracellular reactive oxygen species, as well as a decrease in the concentration of cytosolic calcium and the value of the mitochondrial membrane potential 1 and 2 hours after modification of cells with hydrogen peroxide at a concentration of 10-5 mol/l compared with those for intact immunocytes. It was found that apoptosis of lymphocytes under the influence of exogenous hydrogen peroxide is realized with the participation of receptor-mediated caspase, mitochondrial (with activation of caspase-9) and p53-dependent mechanisms. The protective effect of resveratrol (10-6 mol/l) on lymphocytes after exposure to hydrogen peroxide (10-5 mol / l), which is due to its ability to reduce the level of reactive oxygen species in intact and modified hydrogen peroxide immunocytes, as well as deactivate hydrogen peroxide.
apoptosis, lymphocytes, hydrogen peroxide, caspases
1. Nagata S., Masato Tanaka. Programmed cell death and the immune system. Nature Reviews Immunology, 2017, vol. 17, no. 5, pp. 333-340, doi:https://doi.org/10.1038/nri.2016.153.
2. Savitskaya M.A., Onishchenko G.E. Mechanisms of Apoptosis. Biochemistry (Mosc.), 2015, vol. 80, no. 11, pp. 1393-1405, doi:https://doi.org/10.1134/S0006297915110012.
3. Nakvasina M.A., Artyukhov V.G. Mechanisms of cell death: apoptosis, autophagy, necrosis. Textbook. Voronezh: VSU Publishing House, 2019, 164 p. (In Russ.)
4. Mates J.M., Sanchez-Jimenez F.M. Role of reactive oxygen species in apoptosis: implications for cancer therapy. The International Jornal of Biochemistry & Cell Biology, 2000, vol. 32, pp. 157-170, doi:https://doi.org/10.1016/s1357-2725(99)00088-6.
5. Riazantseva N.V., Novitskii V.V., Chasovskikh N.Iu., Kaigorodova E.V., Starikova E.G., Starikov Iu.V., Radzivil T.T., Krat N.V. The role of redox-dependent signal systems in the regulation of apoptosis under oxidative stress condition. Tsitologiia, 2009, vol. 51, no. 4, pp. 329-334. (In Russ.)
6. Cherenkevich S.N., Martinovich G.G., Martinovich I.V., Gorudko I.V., Shamova E.V. Redox regulation of cellular activity: concepts and mechanisms. Vescі NAN Belarusі. Ser. biyal. Navuk, 2013, vol. 1, pp. 92-108. (In Russ.)
7. Nadeev A.D., Zinchenko V.P., Kudryavtsev I.V., Serebriakova M.K., Avdonin P.V., Goncharov N.V. Dual proapoptptic and pronecrotic effect of hydrogen peroxide on human umbilical vein endothelial cells. Cell and Tissue Biology, 2015, vol. 57, no. 12, pp. 909-916, doi:https://doi.org/10.1134/S1990519X16020097.
8. Artyukhov V.G., Trubitsyna M.S., Nakvasina M.A., Solov′eva E.V., Lidokhova O.V. Ways of realizing apoptosis of human lymphocytes induced by UV-light and reactive oxygen species. Radiats Biol Radioecol, 2011, vol. 51, no. 4, pp. 425-443. (In Russ.)
9. Artyukhov V.G., Trubitsyna M.S., Nakvasina M.A., Solov′eva E.V. DNA Fragmentation of Human Lymphocytes in Dynamics of Development of Apoptosis Induced by Action of UV Radiation and Reactive Oxygen Species. Cell and Tissue Biology, 2011, vol. 5, no. 2, pp. 127-135, doi:https://doi.org/10.1134/S1990519X11020039.
10. Artyukhov V.G., Trubitsyna M.S., Nakvasina M.A., Lidokhova O.V., Ryazantsev S.V. The ways for realization of human lymphocyte apoptosis, induced by hydrogen peroxide. Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii, 2013, no. 7, pp. 7-16. (In Russ.)
11. Nakvasina M.A., Popova L.I., Lidokhova O.V., Artyukhov V.G. Modulation of Structural and Functional Properties of Human Lymphocytes by Reactive Oxygen Species. Bulletin of Experimental Biology and Medicine, 2019. vol. 166, no. 4, pp. 481-486, doi:https://doi.org/10.1007/S10517-019-04377-4.
12. Klaus J. Lymphocytes. Methods. Moscow, Mir, 1990, 395 p. (In Russ.)
13. Kaufmann S., Lee Sun-Hee, Meng X., Loegering D., Kottke T., Heinzing A.J., Ruchaud S., Samejima K., Earnshaw W. Apoptosis-associated caspase activation assays. Methods, 2008, vol. 44, pp. 262-272, doi:https://doi.org/10.1016/j.ymeth.2007.11.005.
14. Hirst R.A., Harrison C., Hirota K., Lambert D.G. Measurement of [Ca²+]i in whole cell suspensions using fura-2. Methods Mol. Biol., 2005, vol. 312, pp. 37-45, doi:https://doi.org/10.1385/1-59259-949-4:037.
15. Rastogi R.P., Singh S.P., Hader D.P., Sinha R.P. Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2',7'-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937. Biochem. Biophys. Res. Commun., 2010, vol. 397, no. 3, pp. 603-607, doi:https://doi.org/10.1016/j.bbrc.2010.06.006.
16. Kudriavtsev I.V., Golovkin A.S., Zurochka A.V., Khaidukov S.V. Modern technologies and approaches to apoptosis studies in experimental biology. Medicinskaya immunologiya, 2012, vol. 14, no. 6, pp. 461-482. (In Russ.)
17. Julien O., Wells I.A. Caspases and their substrates. Cell Death and Differentiation. Advance online publication. 2017, doi: 10/1038/cdd.2017.44.
18. Green D.R., Llambi F. Cell death signaling. Cold Spring Harb Perspect Biol., 2015, doi:https://doi.org/10.1101/cshperspect.a006080.
19. Szegezdi E., Fitzgerald U., Samali A. Caspase-12 and ER-stress-mediated apoptosis: The story so far. Annals of the New York Academy of Sciences, 2003, dec., vol. 1010, pp. 186-194, doi:https://doi.org/10.1196/annals.1299.032.
