В сравнительном аспекте исследованы структурно-функциональные, физико-химические и кинетические свойства инулиназ из различных продуцентов. Проведен анализ методов их иммобилизации, т.е. закрепления на нерастворимых полимерных матрицах очищенных ферментов или ферментных систем, что приводит к стабилизации каталитической активности препаратов и превращает их в технологически более удобные гетерогенные катализаторы. Рассмотрены некоторые пути и перспективы практического использования иммобилизованных инулиназ.
инулиназа, иммобилизация, гетерогенные биокатализаторы
1. Kovaleva T.A., Kholyavka M.G., Artyukhov V.G. Characteristics of inulinases: methods for regulation and stabilization of their activity. Biotechnology in Russia, 2012, no. 1, pp. 43-63.
2. Artyukhov V.G., Holyavka M.G., Kovaleva T.A. Structural and functional properties of inulinases. Ways to regulate their activity. Biophysics, 2013, vol. 58, no. 4, pp. 493-501.
3. Holyavka M.G., Artyukhov V.G., Kovaleva T.A. Structural and functional properties of inulinases: a review. Biocatalysis and Biotransformation, 2016, vol. 34, no. 1, pp. 1-17.
4. Holyavka M.G., Kovaleva T.A., Grechkina M.V., Ostankova I.V., Artyukhov V.G. Inulinases from various producers: the features of their permolecular organization. Applied Biochemistry and Microbiology, 2014, vol. 50, no. 1, pp. 10-16.
5. Holyavka M.G., Artyukhov V.G., Makin S.M. Investigation of inulinase permolecular organization from producers of the genus Aspergillus using several computing and experimental methods. Biophysics, 2015, vol. 60, no. 4, pp. 522-528.
6. Holyavka M.G., Artyukhov V.G., Kovaleva T.A. The handbook of microbial bioresources. Chapter 22. Characteristics of microbial inulinases: physical and chemical bases of their activity regulation / Edited by V.K. Gupta, G.D. Sharma, M.G. Tuohy, R. Gaur, Boston: CABI, 2016, pp. 361-368.
7. Abdullatypov A.V., Kondratyev M.S., Kholyavka M.G., Artyukhov V.G. Reconstruction of the spatial structure of inulinase from Kluyveromyces marxianus to find regulatory pathways of its catalytic activity. Biophysics, 2016, vol. 61, no. 4, pp. 565-571.
8. Holyavka M.G., Kondratyev M.S., Samchenko A.A., Kabanov A.V., Komarov V.M., Artyukhov V.G. In silico design of high-affinity ligands for the immobilization of inulinase. Computers in biology and medicine, 2016, vol. 71, pp. 198-204.
9. Kondratyev M.S., Kholyavka M.G., Kabanov A.V., Sorokin A.A., Artyukhov V.G. In silico design and virtual screening of inulinase immobilization ligands with highest affinity. Journal of biomolecular structure and dynamics, 2015, vol. 33, suppl., pp. 128-129.
10. Kovaleva T.A., Holyavka M.G., Bogdanova S.S. Inulinase immobilization on macroporous anion-exchange resins by different methods. Bulletin of Experimental Biology and Medicine, 2009, vol. 148, no. 1, pp. 39-41.
11. Holyavka M.G., Kovaleva T.A., Karpov S.I., Seredin P.V., Artyukhov V.G. Investigation of mechanisms of interaction between inulinase from Kluyveromyces marxianus and the matrices of ion-exchange resins and fiber. Biophysics, 2014, vol. 59, no. 2, pp. 222-228.
12. Holyavka M.G., Kondratyev M.S., Terentyev V.V., Samchenko A.A., Kabanov A.V., Komarov V.M., Artyukhov V.G. The Molecular Mechanism of Adsorption Immobilization of Inulinase on Polymer Matrices. Biophysics, 2017, vol. 62, no. 1, pp. 5-11.
13. Kovaleva T.A., Kholyavka M.G., Takha A.S. Development of a heterogenous biocatalyst on the basis of immobilized inulinase preparation from Kluyveromyces marxianus. Biotechnology in Russia, 2007, no. 3, pp. 106-116.
14. Kovaleva T.A., Kholyavka M.G., Takha A.S. Study on a few characteristics on immobilized inulinase from Kluyveromyces marxianus as a perspective catalyst for inulin hydrolysis. Biotechnology in Russia, 2009, no. 2, pp. 73-80.
15. Kholyavka M.G., Kovaleva T.A., Khrupina E.A., Volkova S.A., Artyukhov V.G. design of a heterogeneous enzymatic preparation on the basis of immobilized inulinase from Helianthus tuberosus. Biotechnology in Russia, 2012, no. 6, pp. 31-41.
16. Holyavka M.G., Evstigneev M.P., Artyukhov V.G., Savin V.V Development of heterogeneous preparation with inulinase for tubular reactor systems. Journal of Molecular Catalysis B: Enzymatic, 2016, vol. 129, pp. 1-5.
